Jayne was a series regular for four and a half seasons on the acclaimed drama Chicago Hope. Other television credits include series regular roles on John Doe, Imagine That, The District, Sirens, WIOU; recurring roles on Major Crimes, Boston Legal, Private Practice, Brothers and Sisters, Sports Night, Everwood, Off the Map; and guest star roles on Revenge, Castle, Rizolli and Isles, The Cleaner, Eli Stone, Grey’s Anatomy, Without a Trace, and Jack and Bobby. Her television movies include Doing Time on Maple Drive, My Mother the Spy, The Dan Jansen Story, and Trauma. Her feature film credits include Kindergarten Cop, Don’t Tell Mom the Babysitter’s Dead, Clean Slate, Bye Bye Love, Last Dance, Into the Heart, Gattaca and The Sweet Life.

She starred opposite Christine Lahti at the Geffen Playhouse in the LA premiere of Wendy Wasserstein’s play Third and played Goneril in Patsy Rodenberg’s LA directorial debut of Shakespeare’s King Lear. She also starred opposite Didi Conn and Lawrence Pressman in Elliot Shoenman's play A Heap of Livin at the Odyssey Theater in Los Angeles. Jayne appeared on stage in London in Dreams in an Empty City and Every Black Day. Other English stage credits include Much Ado About Nothing, Danny and the Deep Blue Sea, and You’re Gonna Love Tomorrow.

Most recently, Jayne was seen as Starfleet admiral Katrina Cornwell on Star Trek, Discovery and in the short film Sister. Now pivoting to work behind the camera, Jayne wrote, produced, and directed the short film Flip Side and is in development on a proof of concept short for a feature film. She lives in Los Angeles with her husband John Terlesky, with whom she shares two grown daughters.